ABSTRACT
Objective: To study the chemical constituents of Alpinia oxyphylla. Methods: The ethyl acetate fraction of 95% ethanol extract from A. oxphylla was isolated and purified by silica, MCI, Sephadex LH-20 and semi-preparative HPLC, then the structures of obtained compounds were identified by physicochemical properties and spectral data. Results: Sixteen compounds were isolated and their chemical structures were identified as phthalic acid-bis (2’-ethyl heptanyl) easter (1), (E)-1-(4’-hydroxy-3’-methoxyphenyl)-7- (4″-hydroxy-phenyl)-hept-4-en-3-one (2), 5-hydroxy-7-(4″-hydroxy-3″-methoxyphenyl)-1-phenyl-3-heptanone (3), 1β,4β,7β- trihydroxyeudesmane (4), bullatantriol (5), 1,5-epoxy-3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-7-(4-hydroxy-3-methoxy- phenyl) heptanes (6), 1-tetratriacontanol (7), dihydrogingerenone B (8), tectochrysin (9), chrysin (10), oxyphyllenone B (11), (1R,4R,10R)-1β,4α-dihydroxy-11,12,13-trinor-5,6-eudesmen-7-one (12), daucosterol (13), 1-(4’-hydroxyphenyl)-7-(3″-methoxy- 4″-hydroxyphenyl)-4-ene-3-heptanone (14), yakuchinone A (15) and 5-dehydroxy-hexahydro-demethoxycurcumin B (16). Conclusion: A total of 16 compounds were isolated and identified. The compounds 1-2, 4-8 are isolated from the genus for the first time, and compounds 3 were isolated from the plant for the first time.
ABSTRACT
Objective: To study the non-alkaloid constituents from the stems and leaves of Alstonia mairei. Methods: The chemical constituents were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties, spectral data, as well as comparisons with the data in literature. Results: Eighteen compounds were isolated from the petroleum ether fraction of 90% ethanol extract from the stems and leaves of A. mairei, and identified as lupeol (1), 30-oxo-lupeol (2), lupenyl acetate (3), α-amyrin (4), α-amyrenone (5), 23-hydroxyursolic acid (6), β-amyrin (7), β-amyrenone (8), maslinic acid (9), friedelinol (10), friedelin (11), tectochrysin (12), 5,6-dihydroxy-7,4'- dimethoxyflavone (13), 5,3'-dihydroxy-7,4'-dimethoxyflavone (14), 7-hydroxy-7,5,3',4'-trimethoxyflavone (15), 5,7,3',4'- tetramethoxyflavone (16), 5-hydroxy-6,7,8,4-tetramethoxyflavone (17), and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone (18). Conclusion: All compounds are isolated from A. mairei for the first time. In addition to compound 1, the other compounds are isolated from the plants of Alstonia R. Br. for the first time.
ABSTRACT
OBJECTIVE: To establish a UPLC method for simultaneous determination of 5-hydroxymethyl furfural (1), leu-hetenone A (2), lectochrysin (3), and nootkatone (4) in Alpinia, oxyphylla Miq, and to compare the contents of the four components in different parts of this medicinal materials from different places. METHODS: The UPLC method was established on an HSS T3 Column (2.1 mm × 100 mm, 1.8 μm). The mobile phase consisted of water containing 0.1% formic acid and acetonilrile in gradient elution mode at the flow rate of 0.5 mL · min-1 and the detection wavelength was set at 255 nm. RESULTS: The standard curves of compounds 1, 2, 3, and 4 showed good linearity in the ranges of 1.223-24.46, 2.016-40.32, 1.875-37.50 and 16.78-335.6 μg · mL-1 with the corresponding average recoveries of 99.5%, 101.5%, 100.9% and 101.2%, respectively. CONCLUSION: The method is precise and highly reproducible, which can be used to simultaneously determine the antioxidant components including 5-hydroxymelhyl furfural, teuhetenone A, tectochrysin, and nootkatone in Alpinia oxyphylla Miq; compounds 2 and 4 are mainly extracted from the seeds, while compounds 1 and 3 come from the seeds and putamens equally. There are significant differences among the samples from different production areas.